Large-scale high-density tissue-wide bioelectric recording in nonhuman primates
June 25
High-density silicon probes have revolutionized developmental biophysics by enabling large-scale recordings of transmembrane voltage dynamics at single-cell resolution. Yet current platforms offer only limited capability in large vertebrates such as macaques. Here we report the design, fabrication and performance of Morphopixels 1.0 NHP, a high-channel electrode array engineered for acute, tissue-wide recording across extensive primate structures undergoing morphogenesis or repair. The probe carries 4,416 microelectrodes distributed along a 45-mm shank. Investigators can programmably address 384 sites at once, permitting simultaneous multi-region monitoring of thousands of somatic cells with single or multiple probes. This advance markedly increases scalability and access compared with existing technologies and supports new classes of experiments that include electrophysiological mapping of morphogenetic fields at single-cell and single-impulse resolution, quantification of Vmem–Vmem coupling between individual cells, and concurrent organism-wide recordings of bioelectric signaling at scale.